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藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74018PD1/NFAT-Luc/Jurkat

名稱	PD1/NFAT-Luc/Jurkat
型號(hào)	CBP74018
報(bào)價(jià)
特點(diǎn)	PD1/NFAT-Luc/Jurkat，凍存條件：90% FBS+10% DMSO；

詳細(xì)內(nèi)容

I. Background
腫瘤細(xì)胞可以借助免疫檢查點(diǎn)受體逃避機(jī)體免疫系統(tǒng)的識(shí)別和殺傷，因此阻斷免疫檢查點(diǎn)受體可能是一種廣泛有效的治療方法。目前，抗 PD-1/PD-L1 抗體雖然比較成熟，與抗 CTLA4 抗體類似，但由于存在耐藥性，患者的總體有效率較低，因此尋找新的治療靶點(diǎn)迫在眉睫。 Programmed Cell Death Protein 1 (PD-1)，一種在激活的 T 細(xì)胞上表達(dá)的受體，與其配體 PD-L1 和 PD-L2 結(jié)合，負(fù)向調(diào)節(jié)免疫反應(yīng)。 PD-1 配體存在于大多數(shù)癌癥中，PD-1:PD- L1/2 相互作用會(huì)抑制 T 細(xì)胞活性，并使癌細(xì)胞逃避免疫監(jiān)視。 PD1/PDL1 信號(hào)轉(zhuǎn)導(dǎo)通路是腫瘤免疫抑制的重要組成部分，可以抑制 T 淋巴細(xì)胞的興奮，增強(qiáng)腫瘤細(xì)胞的免疫耐受，從而實(shí)現(xiàn)腫瘤免疫逃逸。PD1 與 PDL1 結(jié)合可以減弱 T 細(xì)胞介導(dǎo)的免疫監(jiān)視，導(dǎo)致免疫反應(yīng)缺失，甚至導(dǎo)致 T 細(xì)胞凋亡。PD1/PDL1 抑制劑可解除抗腫瘤 T 細(xì)胞的免疫抑制，從而導(dǎo)致 T 細(xì)胞增殖并滲透到腫瘤微環(huán)境中并誘導(dǎo)抗腫瘤反應(yīng)。PD-1:PD-L1/2 通路還參與調(diào)節(jié) 自身免疫反應(yīng)，使這些蛋白質(zhì)成為多種癌癥以及多發(fā)性硬化癥、關(guān)節(jié)炎、狼瘡和 I 型糖尿病的有希望的治療靶點(diǎn)。磷酸酶 SHP2 是 T 細(xì)胞功能的關(guān)鍵調(diào)節(jié)因子，它介導(dǎo) TCR 下游的激活信號(hào)以及 PD-1 下游的抑制信號(hào)。

II. Description
PD1 NFAT-Luc Jurkat 報(bào)告基因藥靶模型很好的模擬了體內(nèi) PD1&PDL1/PDL2 的信號(hào)轉(zhuǎn) 導(dǎo)過程，原理見下圖所示。 Figure 1. PD1 NFAT-Luc Jurkat 細(xì)胞模型原理圖

III. Introduction
Host Cell:	Jurkat
Expressed gene:	PD1、NFAT-Luciferase
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L, PD1/NFAT, PD-1 NFAT, PD-1/NFAT
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	RPMI-1640+10%FBS+800μg/ml hygromycin+1μg/ml puromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Homo sapiens, human
Tissue:	Peripheral blood
Disease:	Acute T cell leukemia
Morphology:	Lymphoblast
Growth Properties:	Suspension

Ⅴ. Representative Data

Figure 2. Recombinant PD1/NFAT-Luc/Jurkat stably expressing PD1.
Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer).Dose response of Anti-PD-1 Neutralizing Antibody in PD1 NFAT-Luc Jurkat(C16) with PDL1 TCR Activator CHO (C5).

Figure 4. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose response of Anti-PD-1 Neutralizing Antibody in PD1 NFAT-Luc Jurkat(C16) with PDL1 TCR Activator CHO (C5).
Figure 5. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose response of Anti-PD1 Neutralizing Antibody in PD1 NFAT-Luc Jurkat(C16) with PDL2 TCR Activator CHO(C1)..
Figure 6. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose response of PD1 Agonist Antibody in PD1 NFAT-Luc Jurkat(C16) with FcγR aAPC Cell(C1).