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Cell Line Name：

BCR-ABL1 [E255K]/BaF3

Host Cell:

Ba/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

Presence of a BCR-ABL1 fusion gene is necessary for the pathogenesis of CML. In up to 95% of cases, a t(9;22) (q34;q11) translocation results in the BCR-ABL1 fusion gene (Faderl et al. 1999). This translocation results in the Philadephia chromosome. In rare CML cases lacking the traditional t(9;22) translocation, other translocations result in the creation of the BCR-ABL1 fusion gene, which sometimes involve multiple chromosomes.
ABL1 is a tyrosine kinase, and, in normal cells, it plays a role in cellular differentiation and regulation of the cell cycle. The BCR-ABL1 fusion gene creates a constitutively active tyrosine kinase, which leads to uncontrolled proliferation.

1.WB of BCR-ABL1 [E255K]/BaF3

2.Sanger of BCR-ABL1 [E255K]/BaF3

Figure 2. BCR-ABL1 [E255K]/BaF3

Figure 3. BCR-ABL1 [E255K]/BaF3 Fusion

3. Anti-proliferation assay

Figure 4. CTG Proliferation Assay of BaF3 BCR-ABL1 E255K Cells (C3).